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아라카네 야스유키 (Arakane Yasuyuki) 이미지
성명
아라카네 야스유키 (Arakane Yasuyuki) 교수
연구실
곤충키틴생명공학 연구실(농6-407)
교수전공 및 연구분야
곤충분자생물학 및 생화학, 곤충외골격 구조 및 형성 기작 연구
연락처
062-530-0271(연구실)
이메일
arakane@jnu.ac.kr
홈페이지
 
학력

  - 일본, 야마구치 대학교 농학사 (생화학전공) (1987 - 1991)

  - 일본, 도토리 대학교 농학박사 (생화학 및 분자생물학 전공) (1997 - 2000)

경력

  - 2018 ~ 현재: Archives of Insect Biochemistry and Physiology 편집위원

  - 2018 ~ 현재 Entomological Research 편집위원

  - 2012 ~ 2018 Entomological Research 부편집위원장

  - 2015 ~ 현재: 전남대학교, 응용생물학과, 교수

  - 2010 ~ 2015: 전남대학교, 식물생명공학부, 부교수

  - 2005 ~ 2010: 미국, 캔사스주립대학, 연구교수

  - 2004 ~ 2005: 미국, 캔사스주립대학, 박사후연구원

  - 2002 ~ 2004: 미국, 미농무성, 박사후연구원

  - 2001 ~ 2002: 미국, 캔사스주립대학, 박사후연구원

  - 2000 ~ 2001: 일본, 야마구치대학, 생물과학부(Department of Biological Science), 연구원

  - 1991 ~ 2000: 일본, Bankaku So-Honpo 회사, 연구개발부서, 선임연구원

담당교과

- 학부: 곤충생리학, 곤충기능유전체학

- 대학원: 곤충생화학및분자생물학특론, 곤충체벽학, 유전자침묵및기능연구학, 전공영어작성및발표법, 종합해충방제론 

연구분야

   - 곤충의 큐티클/외골격 형성 분자 메커니즘 구명 연구

   - 기능유전체학 기반 키틴 및 큐티클탄닝 대사 구명

   - 곤충 큐티클 형태 및 초미세구조 구명 연구

   - 곤충 특이적 대사 기작 연구 통한 해충 방제 표적 후보 유전자 발굴

연구 내용

Insect cuticle/exoskeleton is an extracellular matrix (ECM) primarily formed from proteins and the polysaccharide chitin. Despite this limited compositional palette, cuticle has remarkably diverse mechanical properties. To comprehensively understand the molecular mechanism underlying the insect cuticle formation, my research has focused on Molecular Biology, Biochemistry and Functional Genomics of the genes involved in two complex pathways, “Chitin metabolism” and “Cuticle tanning (hardening and coloration)”. Because of the absence of chitin in plants and vertebrates, both metabolisms are potential selective targets for controlling pest insects.


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주요 연구과제

- 2018. 3.~ 2021. 2. 키틴대사에 관여하는 효소 및 키틴어셈블리 단백질들의 신규 기능 규명을 통한 곤충의 세포외기질 합성 분자 메커니즘 연구(한국연구재단)

- 2015. 5. ~ 2018. 4. 곤충의 키틴대사기작에 관여하는 유전자의 기능유전체학적 연구(한국연구재단)

- 2012. 5. ~ 2015. 4. 곤충 외골격의 어셈블리 과정에 관여하는 구조 단백질의 신규 기능 규명 연구(한국연구재단)

- 2011. 11. ~ 2012. 11. 해충의 yellow 유전자의 신규기능구명 및 해충 큐티클 맞춤형 방제법 개발 기반연구(농림수산식품기술기획평가원)

- 2011. 9. ~ 2012. 8. 곤충 표피 생합성과정에서 yellow 유전자 기능유전체학적 연구(한국연구재단)

연구 논문

- Mun S, Noh MY, Kramer KJ, Muthukrishnan S, Arakane Y. (2019). Gene functions in adult cuticle pigmentation of the yellow mealworm, Tenebrio molitor. Insect Biochem Mol Biol.

 

- Noh MY, Muthukrishnan S, Kramer KJ, Arakane Y. (2018) A chitinase with two catalytic domains is required for organization of the cuticular extracellular matrix of a beetle. PLoS Genet. 14(3):e1007307.

 

- Noh MY, Muthukrishnan S, Kramer KJ, Arakane Y. (2018) Group I chitin deacetylases are essential for higher order organization of chitin fibers in beetle cuticle. J Biol Chem. 4; 293(18): 6985–6995.

 

- Muthukrishnan S, Arakane Y, Yang Q, Zhang CX, Zhang J, Zhang W, Moussian B. (2018) Future questions in insect chitin biology: A microreview. Arch Insect Biochem Physiol. 98(2):e21454.

 

- Noh MY, Muthukrishnan S, Kramer KJ, Arakane Y. (2017) Development and ultrastructure of the rigid dorsal and flexible ventral cuticles of the elytron of the red flour beetle, Tribolium castaneum. Insect Biochem Mol Biol. 91:21-33.

 

- Noh MY, Koo B, Kramer KJ, Muthukrishnan S, Arakane Y. (2016) Arylalkylamine N-acetyltransferase 1 gene (TcAANAT1) is required for cuticle morphology and pigmentation of the adult red flour beetle, Tribolium castaneum. Insect Biochem Mol Biol. 79:119-129.

 

- Noh MY, Muthukrishnan S, Kramer KJ, Arakane Y. (2016) Cuticle formation and pigmentation in beetles. Curr Opin Insect Sci. 17:1-9.

 

- Mun S, Noh MY, Dittmer NT, Muthukrishnan S, Kramer KJ, Kanost MR, Arakane Y. (2015) Cuticular protein with a low complexity sequence becomes cross-linked during insect cuticle sclerotization and is required for the adult molt. Sci Rep. 21;5:10484.

 

- Chaudhari SS, Noh MY, Moussian B, Specht CA, Kramer KJ, Beeman RW, Arakane Y, Muthukrishnan S. (2015) Knickkopf and retroactive proteins are required for formation of laminar serosal procuticle during embryonic development of Tribolium castaneum. Insect Biochem Mol Biol. 60:1-6.

 

- Noh MY, Muthukrishnan S, Kramer KJ, Arakane Y. (2015) Tribolium castaneum RR-1 cuticular protein TcCPR4 is required for formation of pore canals in rigid cuticle. PLoS Genet. 11(2):e1004963.

 

- Noh MY, Kramer KJ, Muthukrishnan S, Beeman RW, Kanost MR, Arakane Y. (2015) Loss of function of the yellow-e gene causes dehydration-induced mortality of adult Tribolium castaneum. Dev Biol. 15;399(2):315-24.

 

- Peng Z, Green PG, Arakane Y, Kanost MR, Gorman MJ. (2014) A Multicopper Oxidase-Related Protein Is Essential for Insect Viability, Longevity and Ovary Development. PLoS One. 9(10): e111344.

 

- Chaudhari SS, Moussian B, Specht CA, Arakane Y, Kramer KJ, Beeman RW, Muthukrishnan S. (2014) Functional Specialization Among Members Of Knickkopf Family Of Proteins In Insect Cuticle Organization. PLoS Genet. 10(8): e1004537.

 

- Noh MY, Kramer KJ, Muthukrishnan S, Kanost MR, Beeman RW, Arakane Y. (2014) Two major cuticular proteins are required for assembly of horizontal laminae and vertical pore canals in rigid cuticle of Tribolium castaneum. Insect Biochem Mol Biol. 53:22-9.

 

- Mun S, Noh MY, Osanai-Futahashi M, Muthukrishnan S, Kramer KJ, Arakane Y. (2014) A Major Facilitator Superfamily protein encoded by TcMucK gene is not required for cuticle pigmentation, growth and development in Tribolium castaneum. Insect Biochem Mol Biol. 49:43-8.

 

- Chaudhari SS, Arakane Y, Specht CA, Moussian B, Kramer KJ, Muthukrishnan S, Beeman RW. (2013) Retroactive Maintains Cuticle Integrity by Promoting the Trafficking of Knickkopf into the Procuticle of Tribolium castaneum. PLoS Genet. 9(1): e1003268.

 

- Arakane Y, Lomakin J, Gehrke SH, Hiromasa Y, Tomich JM, Muthukrishnan S, Beeman RW, Kramer KJ, Kanost MR. (2012) Formation of Rigid, Non-Flight Forewings (Elytra) of a Beetle Requires Two Major Cuticular Proteins. PLoS Genet. 8(4): e1002682.

 

- Arakane Y, Taira T, Ohnuma T, Fukamizo T. (2012) Chitin-related enzymes in agro-biosciences. Curr Drug Targets. 13(4):442-70.

 

- Gorman MJ, Sullivan LI, Nguyen TD, Dai H, Arakane Y, Dittmer NT, Syed LU, Li J, Hua DH, Kanost MR. (2012) Kinetic properties of alternatively spliced isoforms of laccase-2 from Tribolium castaneum and Anopheles gambiae. Insect Biochem Mol Biol. 42(3): 193–202.

 

- Chaudhari SS, Arakane Y, Specht CA, Moussian B, Boyle DL, Park Y, Kramer KJ, Beeman RW, Muthukrishnan S. (2011) Knickkopf protein protects and organizes chitin in the newly synthesized insect exoskeleton. Proc Natl Acad Sci U S A. 11; 108(41): 17028–17033.

 

- Zhang J, Zhang X, Arakane Y, Muthukrishnan S, Kramer KJ, Ma E, Zhu KY. (2011) Comparative Genomic Analysis of Chitinase and Chitinase-Like Genes in the African Malaria Mosquito (Anopheles gambiae). PLoS One. 6(5): e19899.

 

- Zhang J, Zhang X, Arakane Y, Muthukrishnan S, Kramer KJ, Ma E, Zhu KY. (2011) Identification and characterization of a novel chitinase-like gene cluster (AgCht5) possibly derived from tandem duplications in the African malaria mosquito, Anopheles gambiae. Insect Biochem Mol Biol. 41(8):521-8

 

- Lomakin J, Huber PA, Eichler C, Arakane Y, Kramer KJ, Beeman RW, Kanost MR, Gehrke SH. (2011) Mechanical properties of the beetle elytron, a biological composite material. Biomacromolecules. 14;12(2):321-35.

 

- Arakane Y, Baguinon MC, Jasrapuria S, Chaudhari S, Doyungan A, Kramer KJ, Muthukrishnan S, Beeman RW. (2011) Both UDP N-acetylglucosamine pyrophosphorylases of Tribolium castaneum are critical for molting, survival and fecundity. Insect Biochem Mol Biol. 41(1):42-50.

 

- Lomakin J, Arakane Y, Kramer KJ, Beeman RW, Kanost MR, Gehrke SH. (2010) Mechanical properties of elytra from Tribolium castaneum wild-type and body color mutant strains. J Insect Physiol. 56(12):1901-6.

 

- Arakane Y, Dittmer NT, Tomoyasu Y, Kramer KJ, Muthukrishnan S, Beeman RW, Kanost MR. (2010) Identification, mRNA expression and functional analysis of several yellow family genes in Tribolium castaneum. Insect Biochem Mol Biol. 40(3):259-66.

 

- Jasrapuria S, Arakane Y, Osman G, Kramer KJ, Beeman RW, Muthukrishnan S. (2010) Genes encoding proteins with peritrophin A-type chitin-binding domains in Tribolium castaneum are grouped into three distinct families based on phylogeny, expression and function. Insect Biochem Mol Biol. 40(3):214-27.

 

- Gorman MJ, Arakane Y. (2010) Tyrosine hydroxylase is required for cuticle sclerotization and pigmentation in Tribolium castaneum. Insect Biochem Mol Biol. 40(3): 267–273.

 

- Tomoyasu Y, Arakane Y, Kramer KJ, Denell RE. (2009) Repeated co-options of exoskeleton formation during wing-to-elytron evolution in beetles. Curr Biol. 19(24):2057-65.

 

- Broehan G, Arakane Y, Beeman RW, Kramer KJ, Muthukrishnan S, Merzendorfer H. (2010) Chymotrypsin-like peptidases from Tribolium castaneum: a role in molting revealed by RNA interference. Insect Biochem Mol Biol. 40(3):274-83

 

- Arakane Y, Muthukrishnan S. (2009) Insect chitinase and chitinase-like proteins. Cell Mol Life Sci. 67(2):201-16.

 

- Arakane Y, Lomakin J, Beeman RW, Muthukrishnan S, Gehrke SH, Kanost MR, Kramer KJ. (2009) Molecular and functional analyses of amino acid decarboxylases involved in cuticle tanning in Tribolium castaneum. J Biol Chem. 12;284(24):16584-94.

 

- Arakane Y, Dixit R, Begum K, Park Y, Specht CA, Merzendorfer H, Kramer KJ, Muthukrishnan S, Beeman RW. (2009) Analysis of functions of the chitin deacetylase gene family in Tribolium castaneum. 39(5-6):355-65.

- Arakane Y, Li B, Muthukrishnan S, Beeman RW, Kramer KJ, Park Y. (2008) Functional analysis of four neuropeptides, EH, ETH, CCAP and bursicon, and their receptors in adult ecdysis behavior of the red flour beetle, Tribolium castaneum. Mech Dev. 125(11-12):984-95.

 

- Arakane Y, Specht CA, Kramer KJ, Muthukrishnan S, Beeman RW. (2008) Chitin synthases are required for survival, fecundity and egg hatch in the red flour beetle, Tribolium castaneum. Insect Biochem Mol Biol. 38(10):959-62.

 

- Zhu Q, Arakane Y, Beeman RW, Kramer KJ, Muthukrishnan S. (2008) Functional specialization among insect chitinase family genes revealed by RNA interference. Proc Natl Acad Sci U S A. 105(18):6650-5.

 

- Tribolium Genome Sequencing Consortium. (2008) The genome of the model beetle and pest Tribolium castaneum. Nature. 452(7190):949-55.

 

- Hogenkamp DG, Arakane Y, Kramer KJ, Muthukrishnan S, Beeman RW. (2008) Characterization and expression of the β-N-acetylhexosaminidase gene family of Tribolium castaneum. Insect Biochem Mol Biol. 38(4):478-89.

 

- Zhu Q, Arakane Y, Beeman RW, Kramer KJ, Muthukrishnan S. (2008) Characterization of recombinant chitinase-like proteins of Drosophila melanogaster and Tribolium castaneum. Insect Biochem Mol Biol. 38(4):467-77.

 

- Zhu Q, Arakane Y, Banerjee D, Beeman RW, Kramer KJ, Muthukrishnan S. (2008) Domain organization and phylogenetic analysis of the chitinase-like family of proteins in three species of insects. Insect Biochem Mol Biol. 38(4):452-66.

 

- Dixit R, Arakane Y, Specht CA, Richard C, Kramer KJ, Beeman RW, Muthukrishnan S. (2008) Domain organization and phylogenetic analysis of proteins from the chitin deacetylase gene family of Tribolium castaneum and three other species of insects. Insect Biochem Mol Biol. 38(4):440-51.

 

- Li B, Predel R, Neupert S, Hauser F, Tanaka Y, Cazzamali G, Williamson M, Arakane Y, Verleyen P, Schoofs L, Schachtner J, Grimmelikhuijzen CJ, Park Y. (2008) Genomics, transcriptomics, and peptidomics of neuropeptides and protein hormones in the red flour beetle Tribolium castaneum. Genome Res. 18(1):113-22.

 

- Matsumiya M, Arakane Y, Haga A, Muthukrishnan S, Kramer KJ. (2006) Substrate specificity of chitinases from two species of fish, greenling, Hexagrammos otakii, and common mackerel, Scomber japonicus, and the insect, tobacco hornworm, Manduca sexta. Biosci Biotechnol Biochem. 70(4):971-9.

 

- Bolognesi R, Arakane Y, Muthukrishnan S, Kramer KJ, Terra WR, Ferreira C. (2005) Sequences of cDNAs and expression of genes encoding chitin synthase and chitinase in the midgut of Spodoptera frugiperda. Insect Biochem Mol Biol. 35(11):1249-59.

 

- Arakane Y, Muthukrishnan S, Beeman RW, Kanost MR, Kramer KJ. (2005) Laccase 2 is the phenoloxidase gene required for beetle cuticle tanning. Proc Natl Acad Sci U S A. 102(32):11337-42.

 

- Hogenkamp DG, Arakane Y, Zimoch L, Merzendorfer H, Kramer KJ, Beeman RW, Kanost MR, Specht CA, Muthukrishnan S. (2005) Chitin synthase genes in Manduca sexta: characterization of a gut-specific transcript and differential tissue expression of alternately spliced mRNAs during development. Insect Biochem Mol Biol. 35(6):529-40.

 

- Anand A, Lei Z, Sumner LW, Mysore KS, Arakane Y, Bockus WW, Muthukrishnan S. (2004) Apoplastic extracts from a transgenic wheat line exhibiting lesion-mimic phenotype have multiple pathogenesis-related proteins that are antifungal. Mol Plant Microbe Interact. 17(12):1306-17.

 

- Arakane Y, Hogenkamp DG, Zhu YC, Kramer KJ, Specht CA, Beeman RW, Kanost MR, Muthukrishnan S. (2004) Characterization of two chitin synthase genes of the red flour beetle, Tribolium castaneum, and alternate exon usage in one of the genes during development. Insect Biochem Mol Biol. 34(3):291-304.

 

- Arakane Y, Zhu Q, Matsumiya M, Muthukrishnan S, Kramer KJ. (2003) Properties of catalytic, linker and chitin-binding domains of insect chitinase. Insect Biochem Mol Biol. 33(6):631-48.

 

- Arakane, Y., Hoshika, H., Kawashima, N., Fujiya-Tsujimoto, C., Sasaki, Y. and Koga, D. (2000) Comparison of chitinase isozymes from yam tuber-enzymatic factor controlling the lytic activity of chitinases. Biosci Biotechnol Biochem. 64, 723-730.

 

- Arakane, Y. and Koga, D. (1999) Purification and characterization of a novel chitinase isozyme from yam tuber. Biosci Biotechnol Biochem. 63, 1895-1901.

 

- Koga, D., Yoshioka, T. and Arakane, Y. (1998) HPLC Analysis of Anomeric Formation and Cleavage Pattern by Chitinolytic Enzyme. Biosci Biotechnol Biochem. 62, 1643-1646.

 

- Koga, D., Sasaki, Y., Uchiumi, Y., Hirai, N., Arakane, Y. and Nagamatsu, Y. (1997) Purification and characterization of Bombyx mori chitinases. Insect Biochem Mol Biol. 27, 757-767.

전문학술서적

- Muthukrishnan S, Merzendorfer H, Arakane Y, Yang Q. (2019) Chitin Organizing and Modifying Enzymes and Proteins Involved In Remodeling of the Insect Cuticle. In: Yang Q., Fukamizo T. (eds) Targeting Chitin-containing Organisms. Advances in Experimental Medicine and Biology, vol 1142. Springer. pp 83-114. (ISBN: 978-981-13-7317-6).

 

- Muthukrishnan S, Merzendorfer H, Arakane Y, Yang Q. (2016) Chitin metabolic pathway in insects and their regulation. In: Cohen E, Moussian B, editors. Extracellular composite matrices in arthropods. Switzerland: Springer. pp. 31-65.

 

- Arakane Y, Noh MY, Asano T, Kramer KJ. Tyrosine metabolism for insect cuticle pigmentation and sclerotization. (2016) In: Cohen E, Moussian B, editors. Extracellular composite matrices in arthropods. Switzerland: Springer. pp. 165-220.

 

- Muthukrishnan S, Merzendorfer H, Arakane Y, Kramer KJ. (2012) Chitin metabolism in insects. In: Gilbert LI, editor. Insect molecular biology and biochemistry. Elsevier. pp. 193-235. (ISBN: 978-0-12-384747-8).