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강훈승 교수
연구실
식물분자생물학 연구실(농4-112)
교수전공 및 연구분야
연락처
062-530-2181
이메일
hskang@jnu.ac.kr
홈페이지
 
학력
  • 서울대학교 농학사 (1983)
  • 한국과학기술원 이학석사 (1985)
  • Oregon State University, USA 이학박사 (1993)
경력
  • 2001 ~ 현재: 전남대학교 식물생명공학부 교수
  • 1997 ~ 2001: 금호생명환경과학연구소 전임연구원
  • 1994 ~ 1996: University of California at Berkeley, Post-Doc Researcher
담당교과목
  • 학부 : 생화학, 일반화학, 식물생리학
  • 대학원: 식물생화학, 단백질생화학, 핵산생화학, 식물분자생물학
  • 연구분야
    • 식물 생육 발달 및 스트레스 적응에 관여하는 RNA 대사 연구
    • 엽록체 유전자 발현 조절에 관여하는 RNA 대사 연구
    • 환경 스트레스 적응 기작 규명 및 스트레스 내성 작물 개발 연구
    연구 내용

    본 연구실의 주요 목표는 식물 생장, 발달 및 스트레스 반응에 관여하는 유전자의 전사 후 조절과정을 이해하는 것이다. RNA processing, splicing, turnover 및 translational control 등을 포함한 전사 후 조절은, 다양한 RNA-결합 단백질이 타겟 RNA에 직·간접적으로 결합하여 이루어 진다. 본 연구실은 식물 발달 및 스트레스 반응에 관여하는 RNA-결합 단백질들의 작용 기작을 밝히고, 스트레스 내성 작물을 육성하는 기술을 개발하는 연구를 수행하고 있다. 이러한 목표를 달성하기 위하여, 식물에 존재하는 다양한 RNA-결합 단백질 가운데 glycine-rich RNA-binding proteins, cold shock domain proteins, RNA helicases 등의 기능을 과발현 형질전환체와 돌연변이체를 활용하여 규명하고 있다. 특히, 특정 RNA-결합 단백질이 RNA 샤페론으로 작용하는지를 대장균을 활용한 cold shock assay 및 transcription anti-termination assay, RNA cleavage assay 등을 이용하여 분석하고 있다. 본 연구실은 또한 식물의 스트레스 반응에 관여하는 microRNA의 기능을 규명하는 연구도 수행하고 있다. 이러한 연구 결과를 바탕으로 식물 생육이 양호하며 다양한 스트레스에 내성을 가진 새로운 경제 작물을 개발하는 것이 최종 연구 목표이다.

    연구 논문

    Tao Xu, Yao Chen, and Hunseung Kang (2019). Melatonin is a potential target for improving post-harvest preservation of fruits and vegetables. Front Plant Sci. 10, 1388, 1-14.

     

    Kwanuk Lee, Su Jung Park, Catherine Colas des Francs-Small, Michael Whitby, Ian Small, and Hunseung Kang (2019). The coordinated action of PPR4 and EMB2654 on each intron half mediates trans-splicing of rps12 transcripts in plant chloroplasts. Plant J.

     

    Yeon-Ok Kim, Hunseung Kang, and Sung-Ju Ahn (2019) Overexpression of phytochelatin synthase AtPCS2 enhances salt tolerance in Arabidopsis thaliana. J. Plant Physiol. 240, 1-8.

     

    Kwanuk Lee, Su Jung Park, Youn-Il Park, and Hunseung Kang (2019) CFM9, a mitochondrial CRM protein, is crucial for mitochondrial intron splicing, mitochondria function, and Arabidopsis growth and stress responses. Plant Cell Physiol. 60, 2538-2548.

     

    Kwanuk Lee, Su Jung Park, Ji Hoon Han, Young Jeon, Hyun-Sook Pai, and Hunseung Kang (2019). A chloroplast-targeted pentatricopeptide repeat protein PPR287 is crucial for chloroplast function and Arabidopsis development. BMC Plant Biol. 19, 1-10.

     

    Jianzhong Hu, Stefano Manduzio, and Hunseung Kang (2019). Epitranscriptomic RNA methylation in plant development and abiotic stress responses. Front. Plant Sci. 10, 500, 1-11.

     

    Ghazala Nawaz and Hunseung Kang (2019).Rice OsRH58, a chloroplast DEAD-box RNA helicase, improves salt or drought stress tolerance in Arabidopsis by affecting chloroplast translation. BMC Plant Biol. 19, 17.

     

    Sy Nguyen Dinh, Su Jung Park, Ji Hoon Han, and Hunseung Kang (2019). A chloroplast-targeted S1 RNA-binding domain protein plays a role in Arabidopsis response to diverse abiotic stresses. J. Plant Biol. 62, 74-81.

     

    Ghazala Nawaz, Kwanuk Lee, Su Jung Park, Yeon-Ok Kim, and Hunseung Kang (2018). A chloroplast-targeted cabbage DEAD-box RNA helicase BrRH22 confers abiotic stress tolerance to transgenic Arabidopsis plants by affecting translation of chloroplast transcripts. Plant Physiol. Biochem. 127, 336-342.

     

    Ghazala Nawaz, Than Zaw Tun Sai, Kwanuk Lee, Yeon-Ok Kim, and Hunseung Kang (2018). Rice DEAD-box RNA helicase OsRH53 has negative impact on Arabidopsis response to abiotic stresses. Plant Growth Regulation 85, 153-163.

     

    Hyunjoo Eom, Su Jung Park, Min Kyung Kim, Hoyeun Kim, Hunseung Kang, Ilha Lee (2018). TAF15b, involved in the autonomous pathway for flowering, represses transcription of FLOWERING LOCUS C. Plant J. 93, 79-91.

     

    Sy Nguyen Dinh and Hunseung Kang (2017). An endoplasmic reticulum-localized Coffea arabica BURP domain-containing protein affects the response of transgenic Arabidopsis plants to diverse abiotic stresses. Plant Cell Report 36, 1829-1839.

     

    Ghazala Nawaz, and Hunseung Kang (2017). Chloroplast- or mitochondria- targeted DEAD-box RNA helicases play essential roles in organellar RNA metabolism and abiotic stress responses. Frontier Plant Sci. 8: 871, 1-9.

     

    Kyung Jin Kwak, Bo Mi Kim, Kwanuk Lee, and Hunseung Kang (2017). quatre-quart1 is an indispensable U12 intron-containing gene that plays a crucial role in Arabidopsis development. J. Exp. Bot. 68, 2731-2739.

     

    Jiseong Kim, Tieu-Ngoc Nguyen Le, and Hunseung Kang (2017). Artificial targeting of a nucleus-encoded RNA-binding protein AtRZ1a to chloroplasts affects flowering and ABA response of Arabidopsis thaliana. J. Plant Biol. 60, 278-284.

     

    Kwanuk Lee, Ji Hoon Han, Youn-Il Park, Catherine Colas des Francs-Small, Ian Small, and Hunseung Kang (2017).The mitochondrial pentatricopeptide repeat protein PPR19 is involved in the stabilization of NADH dehydrogenase 1 transcripts and is crucial for mitochondrial function and Arabidopsis thaliana development. New Phytologist 215, 202-216.

     

    Ye Rin Park, Min Ji Choi, Su Jung Park and Hunseung Kang (2017). Three zinc-finger RNA-binding proteins in cabbage (Brassica rapa) play diverse roles in seed germination and plant growth under normal and abiotic stress conditions. Physiol. Plantarum 159, 93-106.

     

    Lili Gu, Hyun Ju Jung, Kyung Jin Kwak, Sy Nguyen Dinh, Yeon-Ok Kim, and Hunseung Kang (2016).AnRRM-containingmei2-like MCT1 plays a negative role in the seed germination and seedling growth of Arabidopsis thaliana in the presence of ABA. Plant Physiol. Biochem. 109, 273-279.

     

    Sy Nguyen Dinh, Than Zaw Tun Sai, Ghazala Nawaz, Kwanuk Lee and Hunseung Kang (2016). Abiotic stresses affect differently the intron splicing and expression of chloroplast genes in coffee plants (Coffea arabica) and rice (Oryza sativa). J. Plant Physiol. 201, 85-94.

     

    Su Jung Park, Hyun Ju Jung, Sy Nguyen Dinh, and Hunseung Kang (2016). Structural features important for the U12snRNA binding and minorspliceosome assembly of Arabidopsis U11/U12-small nuclear ribonucleoproteins. RNA Biology 13, 670-679.

     

    Tao Xu, Bo Mi Kim, Kyung Jin Kwak, Hyun Ju Jung, Hunseung Kang (2016). The Arabidopsis homolog of human minor spliceosomal protein U11-48K plays a crucial role in U12 intron splicing and plant development. J. Exp. Bot. 67 (11), 3397-3406.

     

    Kwanuk Lee, Hunseung Kang (2016). Emerging roles of RNA-binding proteins in plant growth, development, and stress responses. Mol. Cells 39, 179-185.

     

    Kyung Jin Kwak, Hyun-Sung Kim, Ha Young Jang, Hunseung Kang, and Sung-Ju Ahn (2016). Diverse roles of glycine-rich RNA-binding protein7 in the response of camelina (Camelina sativa) to abiotic stress. Acta Physiologia Plantarum 38,, 129.

     

    Lili Gu, Hyun Ju Jung, Bo Mi Kim, Tao Xu, Kwanuk Lee, Yeon-Ok Kim, and Hunseung Kang (2015).Achloroplast-localized S1 domain-containing protein SRRP1 plays a role in Arabidopsis seedling growth in the presence of ABA. J. Plant Physiology 189, 34-41.

     

    Min Ji Choi, Ye Rin Park, Su Jung Park and Hunseung Kang (2015) Stress-responsive expression patterns and functional characterization of cold shock domain proteins in cabbage (Brassica rapa) under abiotic stress conditions. Plant Physiol. Biochem. 96 132-140.

     

    Ji Hoon Han, Kwanuk Lee, Kwang Ho Lee, Sunyo Jung, Young Jeon, Hyun-Sook Pai, and Hunseung Kang (2015) A nuclear-encoded chloroplast-targeted S1 RNA-binding domain protein affects chloroplast rRNA processing and is crucial for normal growth of Arabidopsis thaliana. Plant J. 83, 277-289.

     

    Hwa Jung Lee, Young Ju Park, Kyung Jin Kwak, Donghyun Kim, June Hyun Park, Jae Yun Lim, Chanseok Shin, Kwang-Yeol Yang, and Hunseung Kang (2015) MicroRNA844-guided downregulation of cytidine phosphate diacylglycerol synthase3 (CDS3) mRNA affects the response of Arabidopsis thaliana to bacteria and fungi. Mol. Plant-Microbes Interact. 28, 892-900.

     

    Tao Xu, Hwa Jung Lee, Nguyen Dinh Sy, Hunseung Kang (2015) Wheat (Triticum aestivum) zinc finger-containing glycine-rich RNA-binding protein TaRZ1 affects plant growth and defense response in Arabidopsis thaliana. Plant Growth Regulation 76, 243-250.

    주요 연구과제

    ● 스트레스 내성에 관여하는 RNA 전사체 조절 유전자원 발굴 및 기능 분석


    ● RNA 대사 제어에 의한 엽록체 기능 최적화
    ● 식물 발달에 관여하는 RNA 대사 기능 규명
    Research Area

    The main research focus in my lab is to understand the regulation of gene expression at the posttranscriptional level, which is of crucial importance for growth, development and stress responses of plants. Posttranscriptional gene regulation is achieved by either direct or indirect interactions of RNA-binding proteins to target mRNAs. Our research aims to investigate the functional roles of RNA-binding proteins involved in posttranscriptional regulation and stress adaptation, and to develop potential technologies for generating stress-tolerant crops. To achieve these goals, the functions of RNA-binding proteins, including glycine-rich RNA-binding proteins, cold shock domain proteins and RNA helicases, will be assessed by the analysis of gain-of-function transgenic and loss-of-function mutant plants. In particular, the roles of RNA-binding proteins as RNA chaperones will be investigated by cold shock assay, transcription anti-termination assay and RNA cleavage assay. Our research also aims to understand the functional roles of microRNAs in plant stress responses. The results of the research would provide novel and potential means to develop stress-tolerant crops.