전남대학교

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응용생물학과

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한연수 이미지
성명
한연수 교수
연구실
곤충면역-병리학 연구실(농6-406)
교수전공 및 연구분야
곤충면역-병리학
연락처
062-530-2072
이메일
hanys@jnu.ac.kr
홈페이지
 
학력
  • 순천향대학교 생물학과 이학사 (1984)
  • 고려대학교 생물학과 이학석사 (1986)
  • University of Wisconsin - Madison 이학석사 (1992)
  • University of Wisconsin - Madison 이학박사 (1998)

 

경력
  • 2003 ~ 현재: 전남대학교 응용생물학과 교수
  • 2020 ~ 현재: 친환경농업연구소장 
  • 2011 ~ 2013: 전남대학교 친환경농업연구소 소장
  • 2010 ~ 현재: 기후변화매개체감시거점센터 센터장(질병관리본부)
  • 2009 ~ 2012: 전남대학교 BK21첨단작물보호사업팀 팀장
  • 2007 ~ 현재: Journal of Asia-Pacific Entomology 심사위원
  • 2007 ~ 현재: International Journal of Industrial Entomology 심사위원
  • 2015 ~ 2017: Entomologial Research (SCIE) Editor-in Chief (편집위원장)
  • 2002 ~ 2003: 충북대학교 생명공학연구소 학술연구교수(14-3-3 단백질과 치매)
  • 1998 ~ 2002: Department of Microbiology, Immunology, and Pathology,                                       Colorado State University-Fort Collins  Postdoctoral Fellow(박사후 연구원)

 

담당교과목
    Pest Control
    Molecular Insect Pathology
    Climate Change Vector Biology
    Climate Change Pest Physiology
    Innate Immunity and signaling cascade
    Protein Purification
    Insect Immunology

 

연구분야

Part I : 농작물 생산성 및 병해충 방제 관련 연구

  • 농작물에 발생하는 농해충과 곤충병원성 미생물의 상호작용에 관한 연구
  • 곤충병원성 미생물을 이용한 생물학적 방제에 관한 연구
  • 미생물농법과 연계된 해충밀도 조절 RnD 및 전략개발
  • RNA interference-based pest control 전략개발

 

Part II : 산업곤충(밀웜, Tenebrio molitor)의 Innate Immunity (체액성 및 세포성면역) 연구

  • 밀웜의 유전체(Genome) 및 전사체(Transcriptome) 분석
  • RNAi-based funtional characterization (유전자침묵기법 기반 면역유전자 기능연구)
  • 밀웜의 Spatzle-Toll 신호전달과 항균펩타이 발현 조절기전 연구
  • 밀웜의 IMD/PGRP/IKK 상호작용 연구
  • 밀웜의 Autophagy 유전자 패밀리 발굴 및 기능연구
  • 식용곤충기반 프리미엄(휴먼구레이드) 반려동물간식 수출연구사업단

 

Part III : 사람의 질병을 일으키는 위생 곤충/절지동물 유래 인체바이러스 진단연구

  • 기후변화 매개체감시거점센터(질병관리본부)
  • Flavivirus (뎅기바이러스, 일본뇌염바이러스, 지카바이러스 등) 진단법/진단킷 개발/보급
  • SFTS 바이러스 진단법개발
  • Viral RNA Extraction Kit 개발
  • 식물, 과일, 꿀벌, 모기, 해충 유래 Viral RNA 추출법 최적화 연구

 

연구 내용

 

Part I : 농작물 생산성 및 병해충 방제 관련 연구

  • 농작물에 발생하는 농해충과 곤충병원성 미생물의 상호작용에 관한 연구
  • 곤충병원성 미생물을 이용한 생물학적 방제에 관한 연구
  • 미생물농법과 연계된 해충밀도 조절 RnD 및 전략개발
  • RNA interference-based pest control 전략개발

 

Part II : 산업곤충(밀웜, Tenebrio molitor)의 Innate Immunity (체액성 및 세포성면역) 연구

  • 밀웜의 유전체(Genome) 및 전사체(Transcriptome) 분석
  • RNAi-based funtional characterization (유전자침묵기법 기반 면역유전자 기능연구)
  • 밀웜의 Spatzle-Toll 신호전달과 항균펩타이 발현 조절기전 연구
  • 밀웜의 IMD/PGRP/IKK 상호작용 연구
  • 밀웜의 Autophagy 유전자 패밀리 발굴 및 기능연구
  • 식용곤충기반 프리미엄(휴먼구레이드) 반려동물간식 수출연구사업단

 

Part III : 바이러스추출법 및 진단법 개발

 

  • Flavivirus (뎅기바이러스, 일본뇌염바이러스, 지카바이러스 등) 진단법/진단킷 개발/보급
  • SFTS 바이러스 진단법개발
  • Viral RNA Extraction Kit 개발
  • 식물, 과일, 꿀벌, 모기, 해충 유래 Viral RNA 추출법 최적화 연구

 

연구논문


실험실 대학원생의 논문업적

 

Aedes albopictus Autophagy-Related Gene 8 (AaAtg8) Is Required to Confer Anti-Bacterial Gut Immunity. .  Kim CE, Park KB, Ko HJ, Keshavarz M, Bae YM, Kim B, Patnaik BB, Jang HA, Lee YS, Han YS, Jo YH. Int J Mol Sci. 2020 Apr 22;21(8). pii: E2944. doi: 10.3390/ijms21082944.

 

Keshavarz M, Jo YH, Edosa TT, Han YS.

Insects. 2020 Mar 16;11(3). pii: E188. doi: 10.3390/insects11030188.

 
TmSpz4 Plays an Important Role in Regulating the Production of Antimicrobial Peptides in Response to Escherichia coli and Candida albicans Infections.

Edosa TT, Jo YH, Keshavarz M, Bae YM, Kim DH, Lee YS, Han YS.

 

TmAtg6 Plays an Important Role in Anti-Microbial Defense Against Listeria monocytogenes in the Mealworm, Tenebrio molitor.

Edosa TT, Jo YH, Keshavarz M, Park KB, Cho JH, Bae YM, Kim B, Lee YS, Han YS.

Int J Mol Sci. 2020 Feb 12;21(4). pii: E1232. doi: 10.3390/ijms21041232.

Keshavarz M, Jo YH, Park KB, Ko HJ, Edosa TT, Lee YS, Han YS.

Sci Rep. 2019 Nov 14;9(1):16878. doi: 10.1038/s41598-019-53497-4.

 
Regulation of the expression of nine antimicrobial peptide genes by TmIMD confers resistance against Gram-negative bacteria.

Jo YH, Patnaik BB, Hwang J, Park KB, Ko HJ, Kim CE, Bae YM, Jung WJ, Lee YS, Han YS.

Park S, Jo YH, Park KB, Ko HJ, Kim CE, Bae YM, Kim B, Jun SA, Bang IS, Lee YS, Kim YJ, Han YS.

Front Immunol. 2019 Mar 12;10:310. doi: 10.3389/fimmu.2019.00310. eCollection 2019.


Homologs of Human Dengue-Resistance Genes, FKBP1B and ATCAY, Confer Antiviral Resistance in Aedes aegypti Mosquitoes. Kang S, Shin D, Mathias DK, Londono-Renteria B, Noh MY, Colpitts TM, Dinglasan RR, Han YS, Hong YS.

Insects. 2019 Feb 2;10(2). pii: E46. doi: 10.3390/insects10020046.

 
Molecular Cloning and Effects of Tm14-3-3ζ-Silencing on Larval Survivability Against E. coli and C. albicans in Tenebrio molitor.

 

Seong JH, Jo YH, Seo GW, Park S, Park KB, Cho JH, Ko HJ, Kim CE, Patnaik BB, Jun SA, Choi YS, Kim YW, Bang IS, Lee YS, Han YS.

Genes (Basel). 2018 Jun 29;9(7). pii: E330. doi: 10.3390/genes9070330.


Optimization of double-stranded RNAi intrathoracic injection method in Aedes aegypti.

Kang S, Shin D, Noh MY, Peters JS, Smartt CT, Han YS, Hong YS.

Entomol Res. 2018 Jul;48(4):269-278. doi: 10.1111/1748-5967.12300. Epub 2018 Jul 12.

 
TmSR-C, scavenger receptor class C, plays a pivotal role in antifungal and antibacterial immunity in the coleopteran insect Tenebrio molitor.

 

Kim SG, Jo YH, Seong JH, Park KB, Noh MY, Cho JH, Ko HJ, Kim CE, Tindwa H, Patnaik BB, Bang IS, Lee YS, Han YS.

Insect Biochem Mol Biol. 2017 Oct;89:31-42. doi: 10.1016/j.ibmb.2017.08.007. Epub 2017 Sep 1.

 
TmCactin plays an important role in Gram-negative and -positive bacterial infection by regulating expression of 7 AMP genes in Tenebrio molitor.

 

Jo YH, Kim YJ, Park KB, Seong JH, Kim SG, Park S, Noh MY, Lee YS, Han YS.

Sci Rep. 2017 Apr 18;7:46459. doi: 10.1038/srep46459.

 
The Silencing of a 14-3-3ɛ Homolog in Tenebrio molitor Leads to Increased Antimicrobial Activity in Hemocyte and Reduces Larval Survivability.

 

Seo GW, Jo YH, Seong JH, Park KB, Patnaik BB, Tindwa H, Kim SA, Lee YS, Kim YJ, Han YS.

Genes (Basel). 2016 Aug 20;7(8). pii: E53. doi: 10.3390/genes7080053.

 
Cloning, expression analysis, and RNA interference study of a HORMA domain containing autophagy-related gene 13 (ATG13) from the coleopteran beetle, Tenebrio molitor.

 

Lee JH, Jo YH, Patnaik BB, Park KB, Tindwa H, Seo GW, Chandrasekar R, Lee YS, Han YS.

Front Physiol. 2015 Jun 17;6:180. doi: 10.3389/fphys.2015.00180. eCollection 2015.

 
Molecular cloning and characterization of autophagy-related gene TmATG8 in Listeria-invaded hemocytes of Tenebrio molitor.

 

Tindwa H, Jo YH, Patnaik BB, Lee YS, Kang SS, Han YS.

Dev Comp Immunol. 2015 Jul;51(1):88-98. doi: 10.1016/j.dci.2015.02.017. Epub 2015 Feb 26.

 
Depletion of autophagy-related genes ATG3 and ATG5 in Tenebrio molitor leads to decreased survivability against an intracellular pathogen, Listeria monocytogenes.

 

Tindwa H, Jo YH, Patnaik BB, Noh MY, Kim DH, Kim I, Han YS, Lee YS, Lee BL, Kim NJ.

Arch Insect Biochem Physiol. 2015 Jan;88(1):85-99. doi: 10.1002/arch.21212. Epub 2014 Nov 17.

 
Gene structure, cDNA characterization and RNAi-based functional analysis of a myeloid differentiation factor 88 homolog in Tenebrio molitor larvae exposed to Staphylococcus aureus infection.

 

Patnaik BB, Patnaik HH, Seo GW, Jo YH, Lee YS, Lee BL, Han YS.

Dev Comp Immunol. 2014 Oct;46(2):208-21. doi: 10.1016/j.dci.2014.04.009. Epub 2014 Apr 19.

 
Cloning, characterization and effect of TmPGRP-LE gene silencing on survival of Tenebrio molitor against Listeria monocytogenes infection.

 

Tindwa H, Patnaik BB, Kim DH, Mun S, Jo YH, Lee BL, Lee YS, Kim NJ, Han YS.

Int J Mol Sci. 2013 Nov 14;14(11):22462-82. doi: 10.3390/ijms141122462.

 

Patnaik BB, Kang SM, Seo GW, Lee HJ, Patnaik HH, Jo YH, Tindwa H, Lee YS, Lee BL, Kim NJ, Bang IS, Han YS.

Int J Mol Sci. 2013 Oct 15;14(10):20744-67. doi: 10.3390/ijms141020744.

Kim DH, Patnaik BB, Seo GW, Kang SM, Lee YS, Lee BL, Han YS.

J Invertebr Pathol. 2013 Nov;114(3):226-9. doi: 10.1016/j.jip.2013.08.002. Epub 2013 Aug 17.

 

- Analysis of the genome of a Korean isolate of the Pieris rapae granulovirus enabled by its separation from total host genomic DNA by pulse-field electrophoresis. PLoS One. 2013.

 

Molecular and immunohistochemical characterization of granulin gene encoded in Pieris rapae granulovirus genome. J Invertebr Pathol. 2013 113(1):7-17. 

 

Molecular and immunohistochemical characterization of the chitinase gene from Pieris rapae granulovirus. Arch Virol. 2013 Aug;158(8):1701-18. 

 

Expression analysis and immunohistochemical localization of putative tumor suppressor QM homologue from the cabbage butterfly, Pieris rapae. Entomological Research 2013. 43: 262-270.

 

- Molecular cloning, sequence characterization and expression analysis of a CD63 homologue from the coleopteran beetle, Tenebrio molitor. Int J Mol Sci. 2013 Oct 15;14(10):20744-67. doi: 10.3390/ijms141020744.

 

- Genomic organization, sequence characterization and expression analysis of Tenebrio molitor apolipophorin-III in response to an intracellular pathogen, Listeria monocytogenes. Gene. 2013 Nov 5. doi:pii: S0378-1119(13)01467-4. 10.1016/j.gene.2013.10.058.  

 

- Molecular cloning and characterization of novel Morus alba germin-like protein gene which encodes for a silkworm gut digestion-resistant antimicrobial protein. PLoS One. 2012;7(12):e50900.

 

 

국내외 공동연구 논문업적

- Transcriptome Profile of the Asian Giant Hornet (Vespa mandarinia) Using Illumina HiSeq 4000 Sequencing: De Novo Assembly, Functional Annotation, and Discovery of SSR MarkersInt J Genomics. 2016;2016:4169587. doi: 10.1155/2016/4169587. Epub 2016 Jan 10.


- Understanding regulation of the host-mediated gut symbiont population and the symbiont-mediated host immunity in the Riptortus-Burkholderia symbiosis systemDev Comp Immunol. 2016 Nov;64:75-81. doi: 10.1016/j.dci.2016.01.005. Epub 2016 Jan 13.


- Transcriptome Characterization for Non-Model Endangered Lycaenids, Protantigius superans and Spindasis takanosis, Using Illumina HiSeq 2500 SequencingInt J Mol Sci. 2015 Dec 16;16(12):29948-70. doi: 10.3390/ijms161226213.

 

Intracellular reprogramming of expression, glycosylation, and function of a plant-derived antiviral therapeutic monoclonal antibody. PLoS One. 2013;8(8):e68772. doi: 10.1371/journal.pone.0068772. 

 

- Bahia AC, Kubota MS, Tempone AJ, Araujo HR, Guedes BA, Orfano AS, Tadei WP, Rios-Velasquez CM, Han YS, Secundino NF, Barillas-Mury C, Pimenta PF, Traub-Cseko YM. The JAK-STAT pathway controls Plasmodium vivax load in early stages of Anopheles aquasalis infection. PLoS Negl Trop Dis. 2011 Nov;5(11):e1317. doi: 10.1371/journal.pntd.0001317. 

 

- Park DY, Lee JH, So YK, Kim YK, Ko K, Park SW, Lee YS, Han YS, Ko K. Optimization of expression conditions for production of anti-colorectal cancer monoclonal antibody CO17-1A in baculovirus-insect cell system. Hybridoma (Larchmt). 2011 Oct;30(5):419-26. doi: 10.1089/hyb.2011.0049.

 

- Kim HJ, Kwon YM, Kim YI, Lee IH, Jin BR, Han YS, Cheon HM, Kang YJ, Seo SJ. Molecular cloning and characterization of the STAT gene in Hyphantria cunea haemocytes. Insect Mol Biol. 2011 Dec;20(6):723-32. doi: 10.1111/j.1365-2583.2011.01102.x. 

 

- Wang XF, Zhang BQ, Xu HJ, Cui YJ, Xu YP, Zhang MJ, Han YS, Lee YS, Bao YY, Zhang CX. ODV-associated proteins of the Pieris rapae granulovirus. J Proteome Res. 2011 Jun 3;10(6):2817-27. doi: 10.1021/pr2000804. Epub 2011 May 10.

 

- Kim YI, Kim HJ, Kwon YM, Kang YJ, Lee IH, Jin BR, Han YS, Kim I, Cheon HM, Ha NG, Seo SJ. RNA interference mediated knockdown of apolipophorin-III leads to knockdown of manganese superoxide dismutase in Hyphantria cunea. Comp Biochem Physiol A Mol Integr Physiol. 2011 Jul;159(3):303-12. doi: 10.1016/j.cbpa.2011.03.022. 

 

- Isolation and expression analysis of a homolog of the 14-3-3 epsilon gene in the diamondback moth, Plutella xylostella. Arch Insect Biochem Physiol. 2011 Feb;76(2):114-24. doi: 10.1002/arch.20409.

 

- Gupta L, Noh JY, Jo YH, Oh SH, Kumar S, Noh MY, Lee YS, Cha SJ, Seo SJ, Kim I, Han YS, Barillas-Mury C. Apolipophorin-III mediates antiplasmodial epithelial responses in Anopheles gambiae (G3) mosquitoes. PLoS One. 2010 Nov 2;5(11):e15410. doi: 10.1371/journal.pone.0015410.

 

- Kim YI, Kim HJ, Kwon YM, Kang YJ, Lee IH, Jin BR, Han YS, Cheon HM, Ha NG, Seo SJ. Modulation of MnSOD protein in response to different experimental stimulation in Hyphantria cunea. Comp Biochem Physiol B Biochem Mol Biol. 2010 Dec;157(4):343-50. doi: 10.1016/j.cbpb.2010.08.003. 

 

- Song M, Park DY, Kim Y, Lee KJ, Lu Z, Ko K, Choo YK, Han YS, Ahn MH, Oh DB, Ko K. Characterization of N-glycan structures and biofunction of anti-colorectal cancer monoclonal antibody CO17-1A produced in baculovirus-insect cell expression system. J Biosci Bioeng. 2010 Aug;110(2):135-40. doi: 10.1016/j.jbiosc.2010.01.013

 

-  Kim CH, Shin YP, Noh MY, Jo YH, Han YS, Seong YS, Lee IH. An insect multiligand recognition protein functions as an opsonin for the phagocytosis of microorganisms. J Biol Chem. 2010 Aug 13;285(33):25243-50. doi: 10.1074/jbc.M110.134940. Epub 2010 Jun 2.

 

-  Kwon YM, Kim HJ, Kim YI, Kang YJ, Lee IH, Jin BR, Han YS, Cheon HM, Ha NG, Seo SJ. Comparative analysis of two attacin genes from Hyphantria cunea. Comp Biochem Physiol B Biochem Mol Biol. 2008 Oct;151(2):213-20. doi: 10.1016/j.cbpb.2008.07.002. Epub 2008 Jul 11.

 

-  Lee KS, Kim BY, Kim HJ, Seo SJ, Yoon HJ, Choi YS, Kim I, Han YS, Je YH, Lee SM, Kim DH, Sohn HD, Jin BR. Transferrin inhibits stress-induced apoptosis in a beetle. Free Radic Biol Med. 2006 Oct 1;41(7):1151-61. Epub 2006 Jul 11.

 

-  Kim HJ, Yun CY, Han YS, Lee IH, Kang YJ, Jin BR, Seo SJ. cDNA sequences of two biliproteins, BP1 and BP2, from the cabbage white butterfly, Pieris rapae and their tissue- and stage-specific accumulation. Insect Biochem Mol Biol. 2006 Jan;36(1):54-62. Epub 2005 Nov 10.

 

-  Kim HJ, Je HJ, Cheon HM, Kong SY, Han J, Yun CY, Han YS, Lee IH, Kang YJ, Seo SJ. Accumulation of 23 kDa lipocalin during brain development and injury in Hyphantria cunea. Insect Biochem Mol Biol. 2005 Oct;35(10):1133-41.

 

- Gupta L, Kumar S, Han YS, Pimenta PF, Barillas-Mury C. Midgut epithelial responses of different mosquito-Plasmodium combinations: the actin cone zipper repair mechanism in Aedes aegypti. Proc Natl Acad Sci U S A. 2005 Mar 15;102(11):4010-5. Epub 2005 Mar 7.

 

-  Kim HJ, Je HJ, Park SY, Lee IH, Jin BR, Yun HK, Yun CY, Han YS, Kang YJ, Seo SJ. Immune activation of apolipophorin-III and its distribution in hemocyte from Hyphantria cunea. Insect Biochem Mol Biol. 2004 Oct;34(10):1011-23.

 

-  Kumar S, Gupta L, Han YS, Barillas-Mury C. Inducible peroxidases mediate nitration of anopheles midgut cells undergoing apoptosis in response to Plasmodium invasion. J Biol Chem. 2004 Dec 17;279(51):53475-82. Epub 2004 Sep 29.

 

- Park SY, Kim CH, Jeong WH, Lee JH, Seo SJ, Han YS, Lee IH. Effects of two hemolymph proteins on humoral defense reactions in the wax moth, Galleria mellonella. Dev Comp Immunol. 2005;29(1):43-51.

 

- Kumar S, Christophides GK, Cantera R, Charles B, Han YS, Meister S, Dimopoulos G, Kafatos FC, Barillas-Mury C. The role of reactive oxygen species on Plasmodium melanotic encapsulation in Anopheles gambiae. Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):14139-44. Epub 2003 Nov 17.

 

- Han YS, Barillas-Mury C. Implications of Time Bomb model of ookinete invasion of midgut cells. Insect Biochem Mol Biol. 2002 Oct;32(10):1311-6. Review.

 

- Han YS, Thompson J, Kafatos FC, Barillas-Mury C. Molecular interactions between Anopheles stephensi midgut cells and Plasmodium berghei: the time bomb theory of ookinete invasion of mosquitoes. EMBO J. 2000 Nov 15;19(22):6030-40. Erratum in:EMBO J 2001 Mar 15;20(6):1483. 

 

- Barillas-Mury C, Wizel B, Han YS. Mosquito immune responses and malaria transmission: lessons from insect model systems and implications for vertebrate innate immunity and vaccine development. Insect Biochem Mol Biol. 2000 Jun;30(6):429-42. Review.

 

- Han YS, Chun J, Schwartz A, Nelson S, Paskewitz SM. Induction of mosquito hemolymph proteins in response to immune challenge and wounding. Dev Comp Immunol. 1999 Oct-Dec;23(7-8):553-62.

 

- Barillas-Mury C, Han YS, Seeley D, Kafatos FC. Anopheles gambiae Ag-STAT, a new insect member of the STAT family, is activated in response to bacterial infection. EMBO J. 1999 Feb 15;18(4):959-67.

 

- Han YS, Salazar CE, Reese-Stardy SR, Cornel A, Gorman MJ, Collins FH, Paskewitz SM. Cloning and characterization of a serine protease from the human malaria vector, Anopheles gambiae. Insect Mol Biol. 1997 Nov;6(4):385-95.